TrypLE? Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE? Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Life Technologies offers a variety of TrypLE? formulations for a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE? Express is:

? Gentle on cells
? Room temperature-stable
? Easy to use
? Animal origin-free (AOF)
 

The formulation of TrypLE? solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE? Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room temperature-stable
TrypLE? Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE? Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE? Express, avoiding the need for trypsin inhibitors, such as FBS.

Animal origin-free (AOF)
Unlike porcine trypsin, TrypLE? Express is free of animal-derived components.

cGMP manufacturing and quality system
TrypLE? Express is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

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