human brain tissue were subjected to SDS PAGE followed by western blot with 10944-1-AP (CRH/CRF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 10944-1-AP (CRH/CRF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.