C6 cells expressing ??-galactosidase were grown in 96-well plates. Cells were lysed and assayed using Thermo Scientific M-PER Mammalian Protein Extraction Reagent and Pierce ??-Gal Assay Reagent or corresponding Supplier X reagents. Reactions were stopped with 150?μL of Stop Solution provided in each kit, and absorbances were measured at 405nm using a plate-reading spectrophotometer.
Comparison of beta-galactosidase assay kit protocols
检测: | 报告基因酶 |
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产品线: | Pierce™ |
Substrate Properties: | 化学底物 |
底物类型: | β-Gal 底物 |
描述: | Pierce 哺乳动物 β-半乳糖苷酶检测试剂盒 |
适用于(应用): | Absorbance |
靶标: | Beta-Galactosidase |
数量: | 75 mL 试剂盒 |
标签类型: | 酶标记 |
底物: | 具有专利权 |
免疫测定试剂盒格式: | 96-well Plate |
检测方法: | 比色法 |
适用于(设备): | 微孔板读数仪 |